Rhodopsin Polyclonal Antibody
Ucallm_WP14287-50
Price range: $268.00 through $328.00
Product details
Background:
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form, which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Rhodopsin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 42kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Rhodopsin. AA range:299-348 |
| Specificity | Rhodopsin Polyclonal Antibody detects endogenous levels of Rhodopsin protein. |
| Gene Name | RHO |
| Protein Name | Rhodopsin |
| Other Name | RHO; / OPN2; / Rhodopsin; / Opsin-2 |
| Species | Human |
| Gene ID | 6010 |
| Uniprot | P08100, |
| Species.1 | Mouse |
| Gene ID.1 | 212541 |
| Uniprot.1 | P15409, |
| Species.2 | Rat |
| Gene ID.2 | 24717 |
| Uniprot.2 | P51489 |
| Cellular Localization | Membrane; Multi-pass membrane protein . Cell projection, cilium, photoreceptor outer segment . Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia. . |
| Tissue Expression | Rod shaped photoreceptor cells which mediate vision in dim light. |
| Signaling_pathway | |
| Research Areas | >>Phototransduction |
| Function | Disease:Defects in RHO are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000].,Disease:Defects in RHO are the cause of congenital stationary night blindness autosomal dominant type 1 (CSNBAD1) [MIM:610445]; also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision.,Disease:Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4) [MIM:180380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP4 inheritance is autosomal dominant.,Function:Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.,online information:Retina International's Scientific Newsletter,online information:Rhodopsin entry,online information:Rhodopsin mutations page,PTM:Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.,similarity:Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.,tissue specificity:Rod shaped photoreceptor cells which mediates vision in dim light., |
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