Name: U-Blot® RIP Rabbit mAb
SKU: WR4064
Price: 268 USD
Availability: InStock

U-Blot® RIP Rabbit mAb

WR4064

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4064 Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4064 Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Western blot analysis of lysates from HEK293 cells, using RIP Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4064 Western blot analysis of lysates from HEK293 cells, using RIP Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4064-50

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Product details

Background:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds to the death domain of TNFRSF6 and TRADD. Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RIPK3, UBCE7IP1 isoform 3 (ZIN), EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interacts with MAVS/IPS1.,

Specifications

Size	50μL/100μL
Target	RIP
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	76kD
MW(Observed)	76kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0147R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	RIPK1 RIP RIP1
Protein Name	Receptor-interacting serine/threonine-protein kinase 1 (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP)
Other Name
Species	Human
Gene ID	8737
Uniprot	Q13546,
Species.1	Mouse
Gene ID.1	19766
Uniprot.1	Q60855
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic, Membranous
Tissue Expression	Leukemic T-cell,T-cell,Umbilical vein endothelial cell,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>NF-kappa B signaling pathway; / >>Apoptosis; / >>Necroptosis; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>TNF signaling pathway; / >>Alcoholic liver disease; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Hepatitis C; / >>Human cytomegalovirus infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds to the death domain of TNFRSF6 and TRADD. Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RIPK3, UBCE7IP1 isoform 3 (ZIN), EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interacts with MAVS/IPS1.,