Rock-1 Polyclonal Antibody
Ucallm_WP14359-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015],
Specifications
| Size | 50μL/100μL |
| Target | ROCK1 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | IF, WB, IHC, ELISA |
| MW(Calculated) | |
| MW(Observed) | 158kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ICC 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Rock-1. AA range:262-311 |
| Specificity | Rock-1 Polyclonal Antibody detects endogenous levels of Rock-1 protein. |
| Gene Name | ROCK1 |
| Protein Name | Rho-associated protein kinase 1 |
| Other Name | ROCK1; / Rho-associated protein kinase 1; / Renal carcinoma antigen NY-REN-35; / Rho-associated; / coiled-coil-containing protein kinase 1; / Rho-associated, coiled-coil-containing protein kinase I; / ROCK-I; / p160 ROCK-1; / p160ROCK |
| Species | Human |
| Gene ID | 6093 |
| Uniprot | Q13464, |
| Species.1 | Mouse |
| Gene ID.1 | 19877 |
| Uniprot.1 | P70335, |
| Species.2 | Rat |
| Gene ID.2 | 81762 |
| Uniprot.2 | Q63644 |
| Cellular Localization | Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane; Peripheral membrane protein . Cell projection, bleb . Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Cell projection, ruffle . A small proportion is associated with Golgi membranes (PubMed:12773565). Associated with the mother centriole and an intercentriolar linker (By similarity). Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers (By similarity). Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). . |
| Tissue Expression | Detected in blood platelets. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Sphingolipid signaling pathway; / >>Vascular smooth muscle contraction; / >>TGF-beta signaling pathway; / >>Axon guidance; / >>Focal adhesion; / >>Tight junction; / >>Platelet activation; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Oxytocin signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Yersinia infection; / >>Human cytomegalovirus infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.,enzyme regulation:Activated by RHOA binding.,Function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis.,miscellaneous:Inhibited by Y-27632.,PTM:Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 REM (Hr1) repeat.,subcellular location:Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.,subunit:Binds RHOA (activated by GTP). Interacts with ADD1, GEM, RHOB, RHOC, MYLC2B and VIM (By similarity). Binds RHOE, PPP1R12A, LIMK1 and LIMK2. Interacts with TSG101.,tissue specificity:Detected in blood platelets., |
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