Name: U-Blot® RPA32/RPA2 Rabbit mAb
SKU: WR4505
Price: 268 USD
Availability: InStock

U-Blot® RPA32/RPA2 Rabbit mAb

NIH-3T3 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4505 NIH-3T3 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4505-50

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Product details

Background:function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,

Specifications

Size	50μL/100μL
Target	RPA2
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	29kD
MW(Observed)	32kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0446R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	RPA2
Protein Name	Replication protein A 32 kDa subunit
Other Name	RPA2; / REPA2; / RPA32; / RPA34; / Replication protein A 32 kDa subunit; / RP-A p32; / Replication factor A protein 2; / RF-A protein 2; / Replication protein A 34 kDa subunit; / RP-A p34
Species	Human
Gene ID	6118
Uniprot	P15927,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q62193,
Species.2	Rat
Gene ID.2	59102
Uniprot.2	Q63528
Cellular Localization	Nucleus
Tissue Expression	Brain,Colon,Colon adenocarcinoma,Epithelium,Muscle,Ovary,Pancreas,Placenta,Skin,Tes
Signaling_pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>Ribosome; / >>HIF-1 signaling pathway; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apelin signaling pathway; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Coronavirus disease - COVID-19; / >>Proteoglycans in cancer
Function	Function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,