Name: SIRP-α1 Polyclonal Antibody
SKU: WP14486
Price: 268 USD
Availability: InStock

Product details

Background:

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternati

Specifications

Target	SHPS1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	55kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Sirp alpha1. AA range:451-500
Sequence
Specificity	SIRP-α1 Polyclonal Antibody detects endogenous levels of SIRP-α1 protein.
Gene Name	SIRPA
Protein Name	Tyrosine-protein phosphatase non-receptor type substrate 1
Other Name	SIRPA; / BIT; / MFR; / MYD1; / PTPNS1; / SHPS1; / SIRP; / Tyrosine-protein phosphatase non-receptor type substrate 1; / SHP substrate 1; / SHPS-1; / Brain Ig-like molecule with tyrosine-based activation motifs; / Bit; / CD172 antigen-like family member A; / Inhibito
Species	Human
Gene ID-1	140885
Uniprot	P78324,
Species.1	Mouse
Gene ID-2	19261
Uniprot.1	P97797,
Species.2	Rat
Gene ID-3	25528
Uniprot.2	P97710
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternati
Cellular Localization	Membrane; Single-pass type I membrane protein.
Tissue Expression	Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Signaling_pathway	Organismal Systems >> Development and regeneration >> Osteoclast differentiation
Research Areas	>>Osteoclast differentiation
Function	Function:Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.,PTM:N-glycosylated.,PTM:Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 2 Ig-like C1-type (immunoglobulin-like) domains.,subunit:Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR (By similarity). Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B.,tissue specificity:Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.,
RRID

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SIRP-α1 Polyclonal Antibody

Product details

Specifications

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SIRP-α1 Polyclonal Antibody

Product details

Specifications

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