U-Blot® SIRT3 Rabbit mAb
WR4137 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4137 Immunohistochemical analysis of paraffin-embedded Human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4137 Western blot analysis of lysates from U-14 cells, using SIRT3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | SIRT3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 44kD |
| MW(Observed) | 28kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0250R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SIRT3 |
| Protein Name | NAD-dependent protein deacetylase sirtuin-3 mitochondrial |
| Other Name | SIRT3; / SIR2L3; / NAD-dependent protein deacetylase sirtuin-3; / mitochondrial; / hSIRT3; / Regulatory protein SIR2 homolog 3; / SIR2-like protein 3 |
| Species | Human |
| Gene ID | 23410 |
| Uniprot | Q9NTG7, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8R104 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion matrix |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer |
| Research Areas | >>Nicotinate and nicotinamide metabolism; / >>Metabolic pathways; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,Function:NAD-dependent deacetylase. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.,PTM:Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.,similarity:Belongs to the sirtuin family.,similarity:Contains 1 deacetylase sirtuin-type domain.,tissue specificity:Widely expressed., |
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