U-Blot® SOD2 Rabbit mAb
WR4029
WR4029 Western blot analysis of lysates from Hela cells, using SOD2 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016],
Specifications
| Size | 50μL/100μL |
| Target | SOD2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 25kD |
| MW(Observed) | 22kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0085R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SOD2 |
| Protein Name | Superoxide dismutase [Mn] mitochondrial |
| Other Name | SOD2; / Superoxide dismutase [Mn], mitochondrial |
| Species | Human |
| Gene ID | 6648 |
| Uniprot | P04179, |
| Species.1 | Mouse |
| Gene ID.1 | 20656 |
| Uniprot.1 | P09671, |
| Species.2 | Rat |
| Gene ID.2 | 24787 |
| Uniprot.2 | P07895 |
| Cellular Localization | Mitochondrion matrix |
| Tissue Expression | Brain,Colon,Heart,Liver,Lung,Mammary carcinoma,Tongue, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Neurodegenerative disease >> Huntington disease;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Peroxisome; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Huntington disease; / >>Chemical carcinogenesis - reactive oxygen species; / >>Lipid and atherosclerosis |
| Function | Catalytic activity:2 superoxide + 2 H(+) = O(2) + H(2)O(2).,cofactor:Binds 1 manganese ion per subunit.,Disease:Genetic variation in SOD2 is associated with susceptibility to diabetic nephropathy [MIM:612634]; also called susceptibility to microvascular complications of diabetes type 6 (MVCD6). Diabetic nephropathy is a kidney disease and resultant kidney function impairment due to the long standing effects of diabetes on the microvasculature (glomerulus) of the kidney. Features include increased urine protein and declining kidney function.,Function:Destroys radicals which are normally produced within the cells and which are toxic to biological systems.,online information:Superoxide dismutase entry,online information:The Singapore human mutation and polymorphism database,PTM:Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.,similarity:Belongs to the iron/manganese superoxide dismutase family.,subunit:Homotetramer., |
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