Name: U-Blot® Sodium Potassium ATPase Rabbit mAb
SKU: WR4567
Price: 268 USD
Availability: InStock

Product details

Background:ATPase Na+/K+ transporting subunit alpha 1(ATP1A1) Homo sapiens The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

Specifications

Size	50μL/100μL
Target	Na+/K+-ATPase α1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	113kD
MW(Observed)	100kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:2000-1:10000; WB 1:20000-1:50000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0834R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	ATP1A1
Protein Name	Sodium/potassium-transporting ATPase subunit alpha-1 (Na(+)/K(+) ATPase alpha-1 subunit) (Sodium pump subunit alpha-1)
Other Name	Sodium/potassium-transporting ATPase subunit alpha-1; / Na; / +; / /K; / +; / ATPase alpha-1 subunit; / Sodium pump subunit alpha-1
Species	Human
Gene ID	476
Uniprot	P05023,
Species.1	Mouse
Gene ID.1	11928
Uniprot.1	Q8VDN2,
Species.2	Rat
Gene ID.2	24211
Uniprot.2	P06685
Cellular Localization	Membranous
Tissue Expression
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Cardiac muscle contraction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
Research Areas	>>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Cardiac muscle contraction; / >>Adrenergic signaling in cardiomyocytes; / >>Insulin secretion; / >>Thyroid hormone synthesis; / >>Thyroid hormone signaling pathway; / >>Aldosterone synthesis and secretion; / >>Aldosterone-regulated sodium reabsorption; / >>Endocrine and other factor-regulated calcium reabsorption; / >>Proximal tubule bicarbonate reclamation; / >>Salivary secretion; / >>Gastric acid secretion; / >>Pancreatic secretion; / >>Carbohydrate digestion and absorption; / >>Protein digestion and absorption; / >>Bile secretion; / >>Mineral absorption
Function	Catalytic activity:ATP + H(2)O + Na(+)(In) + K(+)(Out) = ADP + phosphate + Na(+)(Out) + K(+)(In).,Function:This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.,PTM:Phosphorylation on Tyr-10 modulates pumping activity.,similarity:Belongs to the cation transport ATPase (P-type) family.,similarity:Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1.,