Sox2 (Phospho Ser250/Ser251) rabbit pAb
Ucallm_WP10504-50
Price range: $288.00 through $368.00
Product details
Background:
SRY-box 2(SOX2) Homo sapiens This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | SOX-2 Phospho Ser250/251 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 35kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized phosho peptide around human Sox2 (Ser250 and Ser251) |
| Specificity | This antibody detects endogenous levels of SOX-2 only when phosphorylated at Ser250/Ser251,and dually phosphorylated at two sites. |
| Gene Name | SOX2 |
| Protein Name | Sox2 (Ser250/Ser251) |
| Other Name | SOX2; / Transcription factor SOX-2 |
| Species | Human |
| Gene ID | 6657 |
| Uniprot | P48431, |
| Species.1 | Mouse |
| Gene ID.1 | 20674 |
| Uniprot.1 | P48432 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus speckle . Cytoplasm . Nucleus . Acetylation contributes to its nuclear localization and deacetylation by HDAC3 induces a cytoplasmic delocalization (By similarity). Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH) (By similarity). Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus (By similarity). Nuclear import is facilitated by XPO4, a protein that usually acts as a nuclear export signal receptor (By similarity). . |
| Tissue Expression | Fetal brain,Lung,Retina, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway |
| Research Areas | >>Hippo signaling pathway; / >>Signaling pathways regulating pluripotency of stem cells |
| Function | Disease:Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.,Function:Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.,online information:Sox2 entry,PTM:Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.,similarity:Contains 1 HMG box DNA-binding domain., |
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