Price range: $268.00 through $328.00
Background:
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008],
| Size | 50μL/100μL |
| Target | SPG16 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | 69kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human SPG16 AA range: 520-570 |
| Specificity | This antibody detects endogenous levels of SPG16 at Human/Mouse |
| Gene Name | SPAG16 PF20 |
| Protein Name | SPG16 |
| Other Name | |
| Species | Human |
| Gene ID | 79582 |
| Uniprot | Q8N0X2, |
| Species.1 | Mouse |
| Gene ID.1 | 66722 |
| Uniprot.1 | Q8K450 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Cytoplasm, cytoskeleton, flagellum axoneme . Cytoplasm, cytoskeleton, cilium axoneme . Cell projection, cilium, flagellum . Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity). . |
| Tissue Expression | Isoform 1 is detected in testis. Isoform 4 is detected in testis and brain, and at lower levels in kidney, heart, pancreas, thyroid, ovary, adrenal gland, spinal cord, trachea and liver. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Necessary for sperm flagellar function.,similarity:Contains 7 WD repeats.,subcellular location:Detected on the sperm flagellum. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules.,subunit:Interacts with SPAG6.,tissue specificity:Isoform 1 is detected in testis. Isoform 4 is detected in testis and brain, and at lower levels in kidney, heart, pancreas, thyroid, ovary, adrenal gland, spinal cord, trachea and liver., |
