SKU: WP14581 Categories: ,

SRp20 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP14581-100
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Product details

Background:

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010],

Specifications

Size50μL/100μL
TargetSRp20
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)25kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human SFRS3. AA range:111-160
SpecificitySRp20 Polyclonal Antibody detects endogenous levels of SRp20 protein.
Gene NameSRSF3
Protein NameSerine/arginine-rich splicing factor 3
Other NameSRSF3; / SFRS3; / SRP20; / Serine/arginine-rich splicing factor 3; / Pre-mRNA-splicing factor SRP20; / Splicing factor; / arginine/serine-rich 3
SpeciesHuman
Gene ID6428
UniprotP84103,
Species.1Mouse
Gene ID.120383
Uniprot.1P84104
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Nucleus speckle . Cytoplasm . Recruited to nuclear speckles following interaction with YTHDC1. .
Tissue ExpressionBrain,Epithelium,Placenta,Tongue,
Signaling_pathwayHuman Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Research Areas>>Spliceosome; / >>Amyotrophic lateral sclerosis; / >>Herpes simplex virus 1 infection
FunctionFunction:May be involved in RNA processing in relation with cellular proliferation and/or maturation.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with CPSF6, RBMY1A1 and SFRS12.,
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