Name: U-Blot® STAT5 (Phospho Tyr694) Rabbit mAb
SKU: WR4593
Price: 288 USD
Availability: InStock

U-Blot® STAT5 (Phospho Tyr694) Rabbit mAb

THP-1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4593 THP-1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $288.00 through $368.00

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50μl

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SKU: WR4593-50

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Product details

Background:The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been

Specifications

Size	50μL/100μL
Target	STAT5A/B Phospho Tyr694
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	91kD
MW(Observed)	105kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	phosphate
Recommended Dilution Ratio	IHC 1:100-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0067R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	This antibody detects endogenous levels of STAT5A/B only when phosphorylated at Tyr694.This antibody does not recognize phosphorylated at other sites.
Gene Name	STAT5A/STAT5B
Protein Name	Signal transducer and activator of transcription 5A/B
Other Name	STAT5A; / STAT5; / Signal transducer and activator of transcription 5A; / STAT5B; / Signal transducer and activator of transcription 5B
Species	Human
Gene ID	23314
Uniprot	P42229;P51692,
Species.1	Mouse
Gene ID.1	20850;20851,
Uniprot.1
Species.2	Rat
Gene ID.2	24918;25126
Uniprot.2	Q62771;P52632
Cellular Localization	Cytoplasm, Nucleus
Tissue Expression	Brain,Cervix,Epithelium,Lung,Placenta,Synovial membrane
Signaling_pathway
Research Areas
Function	Function:Carries out a dual Function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.,online information:STAT5 entry,PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7.,