Syk (Phospho Ser297) rabbit pAb
Ucallm_WP10516-50
Price range: $288.00 through $368.00
Product details
Background:
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],
Specifications
| Size | 50μL/100μL |
| Target | Syk Phospho Ser297 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 72kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized phosho peptide around human Syk (Ser297) |
| Specificity | This antibody detects endogenous levels of Human Syk (phospho-Ser297) |
| Gene Name | SYK |
| Protein Name | Tyrosine-protein kinase SYK (Ser297) |
| Other Name | SYK; / Tyrosine-protein kinase SYK; / Spleen tyrosine kinase; / p72-Syk |
| Species | Human |
| Gene ID | 6850 |
| Uniprot | P43405, |
| Species.1 | Mouse |
| Gene ID.1 | 20963 |
| Uniprot.1 | P48025, |
| Species.2 | Rat |
| Gene ID.2 | 25155 |
| Uniprot.2 | Q64725 |
| Cellular Localization | Cell membrane . Cytoplasm, cytosol . |
| Tissue Expression | Widely expressed in hematopoietic cells (at protein level) (PubMed:8163536). Expressed in neutrophils (at protein level) (PubMed:15123770). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells (PubMed:8163536). |
| Signaling_pathway | Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>NF-kappa B signaling pathway; / >>Phospholipase D signaling pathway; / >>PI3K-Akt signaling pathway; / >>Osteoclast differentiation; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>C-type lectin receptor signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Tuberculosis; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Coronavirus disease - COVID-19; / >>Viral carcinogenesis |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Function:Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.,PTM:Autophosphorylated.,PTM:Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling.,PTM:Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway.,PTM:Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 SH2 domains.,subunit:Interacts with CBL and SLA when it is phosphorylated. The interaction with SLA may link it to CBL, leading to its destruction. Interacts with phosphorylated NFAM1 (By similarity). Interacts with Epstein-Barr virus LMP2A. Interacts through its SH2 domains with the phosphorylated ITAM domain of CD79A which stimulates SYK autophosphorylation and activation. Interacts with FCRL3., |
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