Syntaxin 1 Polyclonal Antibody
Ucallm_WP15471-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],
Specifications
| Size | 50μL/100μL |
| Target | Syntaxin 1A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human Syntaxin 1A. AA range:31-80 |
| Specificity | Syntaxin 1A Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein. |
| Gene Name | STX1A |
| Protein Name | Syntaxin-1A |
| Other Name | STX1A; / STX1; / Syntaxin-1A; / Neuron-specific antigen HPC-1 |
| Species | Human |
| Gene ID | 6804 |
| Uniprot | Q16623, |
| Species.1 | Mouse |
| Gene ID.1 | 20907 |
| Uniprot.1 | O35526, |
| Species.2 | Rat |
| Gene ID.2 | 116470 |
| Uniprot.2 | P32851 |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . Cell membrane . Colocalizes with KCNB1 at the cell membrane. .; [Isoform 2]: Secreted . |
| Tissue Expression | [Isoform 1]: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex.; [Isoform 2]: Expressed in heart, liver, fat, skeletal muscle, kidney and brain. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Nervous system >> Synaptic vesicle cycle;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>SNARE interactions in vesicular transport; / >>Synaptic vesicle cycle; / >>Insulin secretion; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction |
| Function | Disease:Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,Function:Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.,similarity:Belongs to the syntaxin family.,similarity:Contains 1 t-SNARE coiled-coil homology domain.,subunit:Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.,tissue specificity:Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain., |
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