TDG Polyclonal Antibody
Ucallm_WP14723-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | TDG |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 46kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TDG. AA range:31-80 |
| Specificity | TDG Polyclonal Antibody detects endogenous levels of TDG protein. |
| Gene Name | TDG |
| Protein Name | G/T mismatch-specific thymine DNA glycosylase |
| Other Name | TDG; / G/T mismatch-specific thymine DNA glycosylase; / Thymine-DNA glycosylase |
| Species | Human |
| Gene ID | 6996 |
| Uniprot | Q13569, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P56581 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Colon,Lung,Pooled,Thymus, |
| Signaling_pathway | |
| Research Areas | >>Base excision repair |
| Function | Function:In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.,PTM:Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.,similarity:Belongs to the TDG/mug DNA glycosylase family., |
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