Price range: $268.00 through $328.00
Background:
function:Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase., similarity:Belongs to the TFIIE beta subunit family., similarity:Contains 1 TFIIE beta DNA-binding domain., subunit:Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H.,
| Size | 50μL/100μL |
| Target | TFIIE-β |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 33kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TF2E2. AA range:151-200 |
| Specificity | TFIIE-β Polyclonal Antibody detects endogenous levels of TFIIE-β protein. |
| Gene Name | GTF2E2 |
| Protein Name | Transcription initiation factor IIE subunit beta |
| Other Name | GTF2E2; / TF2E2; / Transcription initiation factor IIE subunit beta; / TFIIE-beta; / General transcription factor IIE subunit 2 |
| Species | Human |
| Gene ID | 2961 |
| Uniprot | P29084, |
| Species.1 | Mouse |
| Gene ID.1 | 68153 |
| Uniprot.1 | Q9D902 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Skin, |
| Signaling_pathway | |
| Research Areas | >>Basal transcription factors; / >>Viral carcinogenesis |
| Function | Function:Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.,similarity:Belongs to the TFIIE beta subunit family.,similarity:Contains 1 TFIIE beta DNA-binding domain.,subunit:Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H., |
