U-Blot® TPST2 Rabbit mAb
WR4754 Western Blot analysis using HepG2 lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene catalyzes the O-sulfation of tyrosine residues within acidic regions of proteins. The encoded protein is a type II integral membrane protein found in the Golgi body. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
Specifications
| Size | 50μL/100μL |
| Target | TPST2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | 41kD |
| MW(Observed) | 41kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1114R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | TPST2 |
| Protein Name | Protein-tyrosine sulfotransferase 2 (EC 2.8.2.20) (Tyrosylprotein sulfotransferase 2) (TPST-2) |
| Other Name | |
| Species | Human |
| Gene ID | 8459 |
| Uniprot | O60704, |
| Species.1 | Mouse |
| Gene ID.1 | 22022 |
| Uniprot.1 | O88856 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:25660941}; Single-pass type II membrane protein {ECO:0000269|PubMed:25660941}. |
| Tissue Expression | TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:9733778, ECO:0000269|PubMed:9736702}. |
| Signaling_pathway | |
| Research Areas | |
| Function | FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate. {ECO:0000269|PubMed:9733778}. |
Please login to post questions
Related products
