U-Blot® TXD12 Rabbit mAb
WR4499 Western Blot analysis using HepG2 lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the thioredoxin superfamily. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. This protein localizes to the endoplasmic reticulum and has a single atypical active motif. The encoded protein is mainly involved in catalyzing native disulfide bond formation and displays activity similar to protein-disulfide isomerases. This protein may play a role in defense against endoplasmic reticulum stress. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Mar 2012],
Specifications
| Size | 50μL/100μL |
| Target | TXD12 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 19kD |
| MW(Observed) | 19kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:500-1:2000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0941R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | TXNDC12 TLP19 UNQ713/PRO1376 |
| Protein Name | TXD12 |
| Other Name | |
| Species | Human |
| Gene ID | 51060 |
| Uniprot | O95881, |
| Species.1 | Mouse |
| Gene ID.1 | 66073 |
| Uniprot.1 | Q9CQU0, |
| Species.2 | Rat |
| Gene ID.2 | 298370 |
| Uniprot.2 | Q498E0 |
| Cellular Localization | Endoplasmic reticulum lumen . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | |
| Research Areas | >>Glutathione metabolism; / >>Metabolic pathways |
| Function | Catalytic activity:2 glutathione + protein-disulfide = glutathione disulfide + protein-dithiol.,cofactor:FAD.,Function:Possesses significant protein thiol-disulfide oxidase activity.,Function:Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). Binds to DNA in a sequence-independent manner.,similarity:Belongs to the FAD-dependent oxidoreductase family.,similarity:Contains 1 thioredoxin domain.,subcellular location:Translocated to the nucleus upon induction of apoptosis.,subunit:Interacts with XIAP.,tissue specificity:Widely expressed., |
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