TyrRS Polyclonal Antibody
Ucallm_WP15759-50
Price range: $268.00 through $328.00
Product details
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | TyrRS |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, ELISA |
| MW(Calculated) | |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:10000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human YARS. AA range:451-500 |
| Specificity | TyrRS Polyclonal Antibody detects endogenous levels of TyrRS |
| Gene Name | YARS |
| Protein Name | TyrRS |
| Other Name | Tyrosine--tRNA ligase, cytoplasmic; / Tyrosyl-tRNA synthetase; / TyrRS |
| Species | Human |
| Gene ID | 8565 |
| Uniprot | P54577, |
| Species.1 | Mouse |
| Gene ID.1 | 107271 |
| Uniprot.1 | Q91WQ3, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q4KM49 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | B-cell lymphoma,Bone,Lung,Platelet, |
| Signaling_pathway | |
| Research Areas | >>Aminoacyl-tRNA biosynthesis |
| Function | Catalytic activity:ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).,Disease:Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.,Function:Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,similarity:Contains 1 tRNA-binding domain.,subunit:Homodimer., |
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