IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality
Monoclonal
Clone Number
RM2037R
Immunogen
The specific immunogen used to produce this antibody is proprietary information.
Specificity
Endogenous
Gene Name
ABHD5 NCIE2 CGI-58
Protein Name
1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (Abhydrolase domain-containing protein 5) (Lipid droplet-binding protein CGI-58)
Other Name
Species
Human
Gene ID
51099
Uniprot
Q8WTS1,
Species.1
Mouse
Gene ID.1
67469
Uniprot.1
Q9DBL9,
Species.2
Rat
Gene ID.2
67469
Uniprot.2
Q6QA69
Cellular Localization
Cytoplasm . Lipid droplet . Cytoplasm, cytosol . Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity). .
Tissue Expression
Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).