Specifications
| Size | 50μL/100μL |
| Target | ACOX1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 74kD |
| MW(Observed) | 74kD(ACOX1),48kD(ACOX1 mature forms) |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1696R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACOX1 ACOX |
| Protein Name | Peroxisomal acyl-coenzyme A oxidase 1 (AOX) (Palmitoyl-CoA oxidase) (Straight-chain acyl-CoA oxidase) (SCOX) |
| Other Name | |
| Species | Human |
| Gene ID | 51 |
| Uniprot | Q15067, |
| Species.1 | Mouse |
| Gene ID.1 | 11430 |
| Uniprot.1 | Q9R0H0, |
| Species.2 | Rat |
| Gene ID.2 | 50681 |
| Uniprot.2 | P07872 |
| Cellular Localization | Peroxisome . |
| Tissue Expression | Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome;Organismal Systems >> Endocrine system >> PPAR signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | |
| Function | Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids . Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)) .; [Isoform 1]: Shows highest activity against medium-chain fatty acyl-CoAs. Shows optimum activity with a chain length of 10 carbons (decanoyl-CoA) in vitro.; [Isoform 2]: Is active against a much broader range of substrates and shows activity towards long-chain fatty acyl-CoAs. |
