Specifications
| Size | 50μL/100μL |
| Target | ALDOA |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 39kD |
| MW(Observed) | 39kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1728R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ALDOA |
| Protein Name | Fructose-bisphosphate aldolase A |
| Other Name | ALDOA; / ALDA; / Fructose-bisphosphate aldolase A; / Lung cancer antigen NY-LU-1; / Muscle-type aldolase |
| Species | Human |
| Gene ID | 226 |
| Uniprot | P04075, |
| Species.1 | Mouse |
| Gene ID.1 | 11674 |
| Uniprot.1 | P05064, |
| Species.2 | Rat |
| Gene ID.2 | 24189 |
| Uniprot.2 | P05065 |
| Cellular Localization | Cytoplasm, myofibril, sarcomere, I band . Cytoplasm, myofibril, sarcomere, M line . In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+). . |
| Tissue Expression | Brain,Cajal-Retzius cell,Cervix,Colon carcinoma,Epithelium,Eye,Feta |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Pentose phosphate pathway; / >>Fructose and mannose metabolism; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of amino acids; / >>HIF-1 signaling pathway |
| Function | Catalytic activity:D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.,Disease:Defects in ALDOA are the cause of aldolase A deficiency [MIM:611881]; also known as aldoA deficiency or red cell aldolase deficiency. Aldolase A deficiency is an autosomal recessive disorder associated with hereditary hemolytic anemia.,miscellaneous:In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.,pathway:Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.,similarity:Belongs to the class I fructose-bisphosphate aldolase family.,subunit:Homotetramer., |
