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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® AMPKγ1 Rabbit mAb
SKU: WR5410 Categories: ,

U-Blot® AMPKγ1 Rabbit mAb

Ucallm_WR5410-50

Price range: $268.00 through $328.00

SKU: WR5410-50
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Product details

Background:The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetAMPKγ1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)38kD
MW(Observed)38kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1659R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NamePRKAG1
Protein Name5'-AMP-activated protein kinase subunit gamma-1
Other NamePRKAG1; / 5'-AMP-activated protein kinase subunit gamma-1; / AMPK gamma1; / AMPK subunit gamma-1; / AMPKg
SpeciesHuman
Gene ID5571
UniprotP54619,
Species.1Mouse
Gene ID.119082
Uniprot.1O54950,
Species.2Rat
Gene ID.225520
Uniprot.2P80385
Cellular Localizationnucleoplasm,cytosol,membrane,nucleotide-activated protein kinase complex,extracellular exosome,
Tissue ExpressionFetal liver,Muscle,Testis,
Signaling_pathwayCellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Organismal Systems >> Environmental adaptation >> Circadian rhythm;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas>>FoxO signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Apelin signaling pathway; / >>Tight junction; / >>Circadian rhythm; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Adipocytokine signaling pathway; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease; / >>Hypertrophic cardiomyopathy
FunctionFunction:AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone-sensitive lipase. This is a regulatory subunit.,similarity:Belongs to the 5'-AMP-activated protein kinase gamma subunit family.,similarity:Contains 4 CBS domains.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic regulatory subunits. Interacts with FNIP1 and FNIP2.,
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