U-Blot® ASC Rabbit mAb
Ucallm_WR6093-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD) . The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells , this protein is localized to the cytoplasm; however , in cells undergoing apoptosis , it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jul 2008] ,
Specifications
| Size | 50μL/100μL |
| Target | ASC |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 22kD |
| MW(Observed) | 24kD |
| Host Species | Rabbit |
| Isotype | IgG, Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2146R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PYCARD |
| Protein Name | Apoptosis-associated speck-like protein containing a CARD |
| Other Name | PYCARD; / ASC; / CARD5; / TMS1; / Apoptosis-associated speck-like protein containing a CARD; / hASC; / Caspase recruitment domain-containing protein 5; / PYD and CARD domain-containing protein; / Target of methylation-induced silencing 1 |
| Species | Human |
| Gene ID | 29108 |
| Uniprot | Q9ULZ3, |
| Species.1 | Mouse |
| Gene ID.1 | 66824 |
| Uniprot.1 | Q9EPB4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm , Nucleus |
| Tissue Expression | Widely expressed at low levels. Detected in peripheral blood leukocytes , lung , small intestine , spleen , thymus , colon and at lower levels in placenta , liver and kidney. Very low expression in skeletal muscle , heart and brain. Expressed in lung epithelial cells (at protein level) (PubMed:23229815) . Detected in the leukemia cell lines HL-60 and U-937 , but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35 , but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway |
| Research Areas | >>Necroptosis; / >>NOD-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>C-type lectin receptor signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Yersinia infection; / >>Influenza A; / >>Lipid and atherosclerosis |
| Function | Domain:Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. ,Function:Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase 9. May be a component of the inflammasome , a protein complex which also includes NALP2 , CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. ,miscellaneous:In breast tumorigenesis , methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death. ,PTM:Phosphorylated. ,similarity:Contains 1 CARD domain. ,similarity:Contains 1 DAPIN domain. ,subcellular location:Upstream of caspase activation , a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow , perinuclear spherical , ball-like structures. ,subunit:Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. ,tissue specificity:Widely expressed at low levels. Detected in peripheral blood leukocytes , lung , small intestine , spleen , thymus , colon and at lower levels in placenta , liver and kidney. Very low expression in skeletal muscle , heart and brain. Detected in the leukemia cell lines HL-60 and U937 , but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35 , but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. , |
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