Name: U-Blot® Bcl-10 Rabbit mAb
SKU: WR5262
Price: 268 USD
Availability: InStock

Product details

Background:This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Specifications

Size	50μL/100μL
Target	Bcl-10
Reactivity	Human
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	26kD
MW(Observed)	32kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1504R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	BCL10
Protein Name	B-cell lymphoma/leukemia 10
Other Name	BCL10; / CIPER; / CLAP; / B-cell lymphoma/leukemia 10; / B-cell CLL/lymphoma 10; / Bcl-10; / CARD-containing molecule enhancing NF-kappa-B; / CARD-like apoptotic protein; / hCLAP; / CED-3/ICH-1 prodomain homologous E10-like regulator; / CIPER; / Cellular homolog
Species	Human
Gene ID	8915
Uniprot	O95999,
Species.1	Mouse
Gene ID.1	12042
Uniprot.1	Q9Z0H7,
Species.2	Rat
Gene ID.2	83477
Uniprot.2	Q9QYN5
Cellular Localization	Cytoplasm, perinuclear region . Membrane raft . Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. .
Tissue Expression	Ubiquitous.
Signaling_pathway	Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway
Research Areas	>>NF-kappa B signaling pathway; / >>C-type lectin receptor signaling pathway; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Shigellosis; / >>Tuberculosis
Function	Disease:A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1; 14)(p22; q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.,Disease:Defects in BCL10 are involved in various types of cancer.,Function:Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.,PTM:Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.,similarity:Contains 1 CARD domain.,subcellular location:Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells.,subunit:Self-associates by CARD-CARD interaction and forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2.,tissue specificity:Ubiquitous.,