U-Blot® BRD9 Recombinant Antibody
UcallM
$999.00
Product details
Background:AbFlex ® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex ® CTCF antibody was expressed in CHO cells and contains rabbit immunoglobulin heavy and light chains. CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. It is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping.
Specifications
| Target | -- |
| Reactivity | Human |
| Applications | WB |
| MW(Calculated) | 85 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Recommended Dilution Ratio | Applications Validated by Active Motif: WB: 0.5µg/mL-2µg/mL |
| Formulation | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein A Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Recombinant |
| Clone Number | -- |
| Immunogen | This antibody was raised against a peptide within the C-terminal region of human BRD9(bromodomain containing 9). |
| Specificity | -- |
| Gene Name | -- |
| Other Name | PRO9856, LAVS3040 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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