SKU: WR5933 Categories: ,

U-Blot® Calpain 1 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5933-50
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Product details

Background:
The calpains , calcium-activated neutral proteases , are nonlysosomal , intracellular cysteine proteases. The mammalian calpains include ubiquitous , stomach-specific , and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large , catalytic subunits associated with a common small , regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme , calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq , Nov 2010] ,

Specifications

Size50μL/100μL
TargetCAN1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)82kD
MW(Observed)75kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1874R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameCAPN1 CANPL1 PIG30
Protein NameCalpain-1 catalytic subunit (Calcium-activated neutral proteinase 1) (CANP 1) (Calpain mu-type) (Calpain-1 large subunit) (Cell proliferation-inducing gene 30 protein) (Micromolar-calpain) (muCANP)
Other Name
SpeciesHuman
Gene ID823
UniprotP07384,
Species.1Mouse
Gene ID.1
Uniprot.1O35350,
Species.2Rat
Gene ID.2
Uniprot.2P97571
Cellular LocalizationCytoplasm . Cell membrane . Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719). .
Tissue ExpressionUbiquitous.
Signaling_pathwayCellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
Research Areas>>Protein processing in endoplasmic reticulum; / >>Apoptosis; / >>Necroptosis; / >>Cellular senescence; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Shigellosis
FunctionCatalytic activity:Broad endopeptidase specificity.,cofactor:Binds 3 calcium ions.,enzyme regulation:Activated by micromolar concentrations of calcium and inhibited by calpastatin.,Function:Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.,online information:Calpain,similarity:Belongs to the peptidase C2 family.,similarity:Contains 1 calpain catalytic domain.,similarity:Contains 4 EF-hand domains.,subcellular location:Translocates to the plasma membrane upon Ca(2+) binding.,subunit:Forms a heterodimer with a small (regulatory) subunit (CAPNS1).,tissue specificity:Ubiquitous.,
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