U-Blot® CHAT Rabbit mAb
Ucallm_WR5177-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010],
Specifications
| Size | 50μL/100μL |
| Target | CHAT |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 83kD |
| MW(Observed) | 83kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1411R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CHAT |
| Protein Name | Choline O-acetyltransferase |
| Other Name | CHAT; / Choline O-acetyltransferase; / CHOACTase; / ChAT; / Choline acetylase |
| Species | Human |
| Gene ID | 1103 |
| Uniprot | P28329, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q03059, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P32738 |
| Cellular Localization | nucleus,cytoplasm,cytosol,presynapse, |
| Tissue Expression | Brain,Lymphocyte,Placenta,Spinal cord, |
| Signaling_pathway | Organismal Systems >> Nervous system >> Cholinergic synapse |
| Research Areas | >>Glycerophospholipid metabolism; / >>Cholinergic synapse |
| Function | Catalytic activity:Acetyl-CoA + choline = CoA + O-acetylcholine.,Disease:Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.,Function:Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.,online information:Choline acetyltransferase entry,similarity:Belongs to the carnitine/choline acetyltransferase family., |
Please login to post questions
Related products
