U-Blot® Cleaved Caspase-1 (Asp297) Rabbit mAb
Ucallm_WR5883-50
Price range: $268.00 through $328.00
Product details
Background：
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits , large and small , that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1 , a cytokine involved in the processes such as inflammation , septic shock , and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq , Mar 2012] ,
Specifications
| Size | 50μL/100μL |
| Target | Caspase-1 |
| Reactivity | Human |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 45kD |
| MW(Observed) | 20,22kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1368R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CASP1 IL1BC IL1BCE |
| Protein Name | Caspase-1 |
| Other Name | CASP1; / IL1BC; / IL1BCE; / Caspase-1; / CASP-1; / Interleukin-1 beta convertase; / IL-1BC; / Interleukin-1 beta-converting enzyme; / ICE; / IL-1 beta-converting enzyme; / p45; / [Cleaved into: Caspase-1 subunit p20; / Caspase-1 subunit p10] |
| Species | Human |
| Gene ID | 834 |
| Uniprot | P29466, |
| Species.1 | Mouse |
| Gene ID.1 | 12362 |
| Uniprot.1 | P29452, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P43527 |
| Cellular Localization | Cytoplasm . Cell membrane . |
| Tissue Expression | Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis |
| Research Areas | >>Necroptosis; / >>Neutrophil extracellular trap formation; / >>NOD-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>C-type lectin receptor signaling pathway; / >>Amyotrophic lateral sclerosis; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Yersinia infection; / >>Influenza A; / >>Coronavirus disease - COVID-19; / >>Lipid and atherosclerosis |
| Function | Alternative products:Additional isoforms seem to exist,Catalytic activity:Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.,enzyme regulation:Specifically inhibited by the cowpox virus Crma protein.,Function:Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.,PTM:The two subunits are derived from the precursor sequence by an autocatalytic mechanism.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18.,tissue specificity:Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain., |
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