U-Blot® DNA Ligase III Rabbit mAb
Ucallm_WR5228-50
Price range: $268.00 through $328.00
Product details
Background:This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | DNA Ligase III |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 113kD |
| MW(Observed) | 102kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:200; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1465R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | LIG3 |
| Protein Name | DNA ligase 3 |
| Other Name | LIG3; / DNA ligase 3; / DNA ligase III; / Polydeoxyribonucleotide synthase [ATP] 3 |
| Species | Human |
| Gene ID | 3980 |
| Uniprot | P49916, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P97386 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | [Isoform 1]: Mitochondrion . Contains an N-terminal mitochondrial transit peptide. .; [Isoform 2]: Mitochondrion . Contains an N-terminal mitochondrial transit peptide. .; [Isoform 3]: Nucleus . Lacks the N-terminal mitochondrial transit peptide. .; [Isoform 4]: Nucleus . Lacks the N-terminal mitochondrial transit peptide. . |
| Tissue Expression | Testis, thymus, prostate and heart. |
| Signaling_pathway | |
| Research Areas | >>Base excision repair |
| Function | Catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,Function:Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.,online information:DNA ligase entry,similarity:Belongs to the ATP-dependent DNA ligase family.,similarity:Contains 1 BRCT domain.,similarity:Contains 1 PARP-type zinc finger.,tissue specificity:Testis, thymus, prostate and heart., |
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