Price range: $268.00 through $328.00
Background:This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | eEF2K |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 85kD |
| MW(Observed) | 105kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1420R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | EEF2K |
| Protein Name | Eukaryotic elongation factor 2 kinase |
| Other Name | EEF2K; / Eukaryotic elongation factor 2 kinase; / eEF-2 kinase; / eEF-2K; / Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
| Species | Human |
| Gene ID | 29904 |
| Uniprot | O00418, |
| Species.1 | Mouse |
| Gene ID.1 | 13631 |
| Uniprot.1 | O08796, |
| Species.2 | Rat |
| Gene ID.2 | 25435 |
| Uniprot.2 | P70531 |
| Cellular Localization | cytoplasm,cytosol,postsynaptic density, |
| Tissue Expression | Epithelium,Glial tumor,Lymph,T-cell, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>AMPK signaling pathway; / >>Oxytocin signaling pathway |
| Function | Catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,Function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer ., |
