Specifications
| Size | 50μL/100μL |
| Target | ERK 3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 83kD |
| MW(Observed) | 105kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1786R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MAPK6 |
| Protein Name | Mitogen-activated protein kinase 6 |
| Other Name | MAPK6; / ERK3; / PRKM6; / Mitogen-activated protein kinase 6; / MAP kinase 6; / MAPK 6; / Extracellular signal-regulated kinase 3; / ERK-3; / MAP kinase isoform p97; / p97-MAPK |
| Species | Human |
| Gene ID | 5597 |
| Uniprot | Q16659, |
| Species.1 | Mouse |
| Gene ID.1 | 50772 |
| Uniprot.1 | Q61532, |
| Species.2 | Rat |
| Gene ID.2 | 58840 |
| Uniprot.2 | P27704 |
| Cellular Localization | Cytoplasm . Nucleus . Translocates to the cytoplasm following interaction with MAPKAPK5. . |
| Tissue Expression | Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. |
| Signaling_pathway | Organismal Systems >> Immune system >> IL-17 signaling pathway |
| Research Areas | >>IL-17 signaling pathway |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation.,Function:Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.,PTM:Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts., |
