Name: U-Blot® GPX3 Rabbit mAb
SKU: WR5378
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designa

Specifications

Target	GPX3
Reactivity	Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	26kD
MW(Observed)	20kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	PT1622R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Sequence
Specificity	Endogenous
Gene Name	GPX3 GPXP
Protein Name	Glutathione peroxidase 3 (GPx-3) (GSHPx-3) (Extracellular glutathione peroxidase) (Plasma glutathione peroxidase) (GPx-P) (GSHPx-P)
Other Name
Species	Human
Gene ID-1	2878
Uniprot	P22352,
Species.1	Mouse
Gene ID-2
Uniprot.1	P46412,
Species.2	Rat
Gene ID-3
Uniprot.2	P23764
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designa
Cellular Localization	Secreted.
Tissue Expression	Secreted in plasma.
Signaling_pathway	Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
Research Areas	>>Glutathione metabolism; / >>Arachidonic acid metabolism; / >>Metabolic pathways; / >>Thyroid hormone synthesis; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases
Function	Catalytic activity:2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O.,Function:Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.,online information:Glutathione peroxidase entry,PTM:The N-terminus is blocked.,similarity:Belongs to the glutathione peroxidase family.,subunit:Homotetramer.,tissue specificity:Secreted in plasma.,
RRID