U-Blot® Histone H3 (Tri Methyl Lys27, Phospho Ser28) Rabbit pAb
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Price range: $380.00 through $950.00
Product details
Background:Histones are basic nuclear proteins that assemble into nucleosomes, the fundamental repeating units of eukaryotic chromatin. Multiple post‑translational modifications can coexist on the same histone H3 N‑terminal tail, forming combinatorial epigenetic signals. H3K27me3S28p represents the dual modification of trimethyl‑Lysine 27 and phosphorylated‑Serine 28 on histone H3. H3K27me3 is deposited by Polycomb Repressive Complex 2 (PRC2), marking facultative heterochromatin and mediating developmental gene silencing. During mitosis, Aurora‑B kinase catalyzes phosphorylation at Ser28 on H3K27me3‑bearing histones.
Specifications
| Target | Histone H3 |
| Reactivity | Human |
| Application | ChIP,ChIP-Seq,WB,DB,ELISA |
| MW(Calculated) | 15kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | |
| Conjugate | Unconjugated |
| Modification | Tri Methyl, Phospho |
| Modification site | Lys27, Ser28 |
| Recommended dilution ratio | ChIP/ChIP-seq 1-2 μg/ChIP; ELISA 1:200; DB 1:2,000; WB 1:1,000 |
| Formulation | PBS containing 0.05% azide and 0.05% ProClin 300 |
| Source | |
| Purification | Affinity purified |
| Purity | |
| Storage | Store at -20°C. Avoid freeze/thaw cycles. |
| Concentration | 0.17 µg/µl |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | KLH-conjugated synthetic peptide |
| Sequence | |
| Specificity | |
| Gene name | |
| Protein name | |
| Alternative name | H3K27me3S28p |
| Organism-1 | |
| Gene ID‑1 | |
| SwissProt-1 | |
| Organism-2 | |
| Gene ID‑2 | |
| SwissProt-2 | |
| Organism-3 | |
| Gene ID‑3 | |
| SwissProt-3 | |
| Organism-4 | |
| Gene ID‑4 | |
| SwissProt-4 | |
| Cellular Localization | |
| Unnamed: 38 | |
| Unnamed: 39 |
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