U-Blot® Histone H3 (Tri Methyl Lys36) Rabbit pAb
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Price range: $700.00 through $1,750.00
Product details
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
Specifications
| Target | Histone H3 |
| Reactivity | Human, Mouse, Wide Range Predicted |
| Application | CUT&RUN,CUT&Tag,ChIP,ChIP-Seq,WB |
| MW(Calculated) | |
| MW(Observed) | 17 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Modification | Tri Methyl |
| Modification site | Lys36 |
| Recommended dilution ratio | ChIP 10 µg per ChIP; ChIP-Seq 4 µg each; WB 0.5 - 2 µg/ml dilution; CUT&Tag 1 µg per 50 µl reaction |
| Formulation | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| Source | |
| Purification | Protein A Chromatography |
| Purity | |
| Storage | -20°C / 2 year. Avoid freeze/thaw cycles. |
| Concentration | 1 µg/µl |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3. |
| Sequence | |
| Specificity | |
| Gene name | HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B |
| Protein name | Histone H3.1/Histone H3.2/Histone H3.3 |
| Alternative name | H3K36ME3;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST2H3A;HIST2H3C;H3F2;H3FM;HIST2H3D;Histone H3.2;Histone H3/m;Histone H3/o;H3F3A;H3.3A;H3F3;PP781;H3F3B;H3.3B;Histone H3.3 |
| Organism-1 | Human |
| Gene ID‑1 | 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968 |
| SwissProt-1 | P68431;Q71DI3;P84243 |
| Organism-2 | Mouse |
| Gene ID‑2 | 319152;15077;15078 |
| SwissProt-2 | |
| Organism-3 | Rat |
| Gene ID‑3 | 291159;100361558 |
| SwissProt-3 | Q6LED0;P84245 |
| Organism-4 | |
| Gene ID‑4 | |
| SwissProt-4 | |
| Cellular Localization | |
| Unnamed: 38 | |
| Unnamed: 39 |
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