Specifications
| Size | 50μL/100μL |
| Target | HNF1A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 67kD |
| MW(Observed) | 81kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1829R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HNF1A TCF1 |
| Protein Name | Hepatocyte nuclear factor 1-alpha (HNF-1-alpha) (HNF-1A) (Liver-specific transcription factor LF-B1) (LFB1) (Transcription factor 1) (TCF-1) |
| Other Name | Hepatocyte nuclear factor 1-alpha; / HNF-1-alpha; / HNF-1A; / Liver-specific transcription factor LF-B1; / LFB1; / Transcription factor 1; / TCF-1 |
| Species | Human |
| Gene ID | 6927 |
| Uniprot | P20823, |
| Species.1 | Mouse |
| Gene ID.1 | 21405 |
| Uniprot.1 | P22361, |
| Species.2 | Rat |
| Gene ID.2 | 24817 |
| Uniprot.2 | P15257 |
| Cellular Localization | Nucleus . |
| Tissue Expression | Liver. |
| Signaling_pathway | |
| Research Areas | >>Maturity onset diabetes of the young |
| Function | Disease:Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].,Disease:Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications.,Disease:Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas.,Function:Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.,online information:Hepatocyte nuclear factors entry,polymorphism:The Ala-98/Val-98 polymorphism is associated with a reduction in glucose-induced serum C-peptide and insulin responses.,similarity:Belongs to the HNF1 homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,subunit:Binds DNA as a dimer.,tissue specificity:Liver., |
