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Background:The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015],
| Size | 50μL/100μL |
| Target | IL-33 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 31kD |
| MW(Observed) | 35kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1553R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | IL33 |
| Protein Name | Interleukin-33 |
| Other Name | IL33; / C9orf26; / IL1F11; / NFHEV; / Interleukin-33; / IL-33; / Interleukin-1 family member 11; / IL-1F11; / Nuclear factor from high endothelial venules; / NF-HEV |
| Species | Human |
| Gene ID | 90865 |
| Uniprot | O95760, |
| Species.1 | Mouse |
| Gene ID.1 | 77125 |
| Uniprot.1 | Q8BVZ5, |
| Species.2 | Rat |
| Gene ID.2 | 361749 |
| Uniprot.2 | Q66H70 |
| Cellular Localization | Nucleus . Chromosome . Cytoplasm . Cytoplasmic vesicle, secretory vesicle . Secreted . Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). . |
| Tissue Expression | Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction |
| Research Areas | >>Cytokine-cytokine receptor interaction; / >>Necroptosis; / >>Cytosolic DNA-sensing pathway; / >>Influenza A |
| Function | Caution:Was originally (PubMed:12819012) thought to one of the key factors that controls the specialized post-capillary high endothelial venules (HEV) phenotype found in organized secondary lymphoid tissue and to be nuclear localized.,Function:Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.,PTM:Proteolytically converted to a mature form by CASP1.,similarity:Belongs to the IL-1 family. Highly divergent.,tissue specificity:Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta., |
