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Background:This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7. [provided by RefSeq, Jul 2013],
| Target | LYPLA1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 25kD |
| MW(Observed) | 24kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | PT1669R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Sequence | |
| Specificity | Endogenous |
| Gene Name | LYPLA1 |
| Protein Name | Acyl-protein thioesterase 1 |
| Other Name | LYPLA1; / APT1; / LPL1; / Acyl-protein thioesterase 1; / APT-1; / hAPT1; / Lysophospholipase 1; / Lysophospholipase I; / LPL-I; / LysoPLA I |
| Species | Human |
| Gene ID-1 | 10434 |
| Uniprot | O75608, |
| Species.1 | Mouse |
| Gene ID-2 | 18777 |
| Uniprot.1 | P97823, |
| Species.2 | Rat |
| Gene ID-3 | 25514 |
| Uniprot.2 | P70470 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7. [provided by RefSeq, Jul 2013], |
| Cellular Localization | Cytoplasm . Cell membrane . Nucleus membrane . Endoplasmic reticulum . Shows predominantly a cytoplasmic localization with a weak expression in the cell membrane, nuclear membrane and endoplasmic reticulum. . |
| Tissue Expression | Platelets. |
| Signaling_pathway | |
| Research Areas | >>Glycerophospholipid metabolism; / >>Choline metabolism in cancer |
| Function | Catalytic activity:Palmitoyl-protein + H(2)O = palmitate + protein.,Function:Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Also has low lysophospholipase activity.,similarity:Belongs to the AB hydrolase 2 family.,subunit:Homodimer., |
| RRID |
