Name: U-Blot® JAM-A Rabbit mAb
SKU: WR5333
Price: 268 USD
Availability: InStock

Product details

Background:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008],

Specifications

Target	JAM-A
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	33kD
MW(Observed)	38kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	PT1567R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Sequence
Specificity	Endogenous
Gene Name	F11R
Protein Name	Junctional adhesion molecule A
Other Name	F11R; / JAM1; / JCAM; / Junctional adhesion molecule A; / JAM-A; / Junctional adhesion molecule 1; / JAM-1; / Platelet F11 receptor; / Platelet adhesion molecule 1; / PAM-1; / CD321
Species	Human
Gene ID-1	50848
Uniprot	Q9Y624,
Species.1	Mouse
Gene ID-2	16456
Uniprot.1	O88792,
Species.2	Rat
Gene ID-3	116479
Uniprot.2	Q9JHY1
Organism-4
Gene ID-4
SwissProt-4
Background	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008],
Cellular Localization	Cell junction, tight junction . Cell membrane; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells. .
Tissue Expression	Expressed in endothelium, epithelium and leukocytes (at protein level).
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Cell adhesion molecules; / >>Tight junction; / >>Leukocyte transendothelial migration; / >>Epithelial cell signaling in Helicobacter pylori infection
Function	Function:Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.,PTM:N-glycosylated.,similarity:Belongs to the immunoglobulin superfamily.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,subcellular location:Localized at tight junctions of both epithelial and endothelial cells.,subunit:Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with the orthoreovirus sigma-1 capsid protein.,
RRID