Name: U-Blot® Ku-70 Rabbit mAb
SKU: WR5285
Price: 268 USD
Availability: InStock

Product details

Background:The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Ku70/XRCC6
Reactivity	Human
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	70kD
MW(Observed)	70kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1547R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	XRCC6
Protein Name	X-ray repair cross-complementing protein 6
Other Name	XRCC6; / G22P1; / X-ray repair cross-complementing protein 6; / 5'-deoxyribose-5-phosphate lyase Ku70; / 5'-dRP lyase Ku70; / 70 kDa subunit of Ku antigen; / ATP-dependent DNA helicase 2 subunit 1; / ATP-dependent DNA helicase II 70 kDa subunit; / CTC box-
Species	Human
Gene ID	2547
Uniprot	P12956,
Species.1	Mouse
Gene ID.1	14375
Uniprot.1	P23475
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus . Chromosome .
Tissue Expression	Brain,Cervix carcinoma,Epithelium,Heart,Hepatocyte,Kidney,Liver,Lun
Signaling_pathway
Research Areas	>>Non-homologous end-joining
Function	developmental stage:Expression does not increase during promyelocyte differentiation.,Disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of p70 containing the leucine repeat. The majority of autoantibodies to p70 in most sera from patients with SLE seem to be reactive with this region.,Function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression.,induction:In osteoblasts, by FGF2.,PTM:Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.,similarity:Belongs to the ku70 family.,similarity:Contains 1 Ku domain.,similarity:Contains 1 SAP domain.,subunit:Heterodimer of a 70 kDa and a 80 kDa subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NARG1, and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, the 70 kDa subunit binds to the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5. Interacts with ELF3. Interactis with XRCC6BP1.,