U-Blot® MGST1 Rabbit mAb
Ucallm_WR5422-50
Price range: $268.00 through $328.00
Product details
Background:The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012],
Specifications
| Size | 50μL/100μL |
| Target | MGST1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 18kD |
| MW(Observed) | 15kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1671R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MGST1 |
| Protein Name | Microsomal glutathione S-transferase 1 |
| Other Name | MGST1; / GST12; / MGST; / Microsomal glutathione S-transferase 1; / Microsomal GST-1; / Microsomal GST-I |
| Species | Human |
| Gene ID | 4257 |
| Uniprot | P10620, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q91VS7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Microsome . Mitochondrion outer membrane; Peripheral membrane protein . Endoplasmic reticulum membrane; Multi-pass membrane protein . |
| Tissue Expression | Highly expressed in liver. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma |
| Research Areas | >>Glutathione metabolism; / >>Metabolism of xenobiotics by cytochrome P450; / >>Drug metabolism - cytochrome P450; / >>Drug metabolism - other enzymes; / >>Metabolic pathways; / >>Platinum drug resistance; / >>Pathways in cancer; / >>Chemical carcinogenesis - DNA adducts; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Hepatocellular carcinoma; / >>Fluid shear stress and atherosclerosis |
| Function | Catalytic activity:RX + glutathione = HX + R-S-glutathione.,enzyme regulation:Activated by N-ethylmaleimide, except in the testis.,Function:Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.,similarity:Belongs to the MAPEG family.,subunit:Homotrimer.,tissue specificity:Highly expressed in liver., |
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