U-Blot® Muscarinic Acetylcholine Receptor M2 Rabbit mAb
Ucallm_WR5757-50
Price range: $268.00 through $328.00
Specifications
| Size | 50μL/100μL |
| Target | Muscarinic acetylcholine receptor M2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 52kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1719R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CHRM2 |
| Protein Name | Muscarinic acetylcholine receptor M2 |
| Other Name | CHRM2; / Muscarinic acetylcholine receptor M2 |
| Species | Human |
| Gene ID | 1129 |
| Uniprot | P08172, |
| Species.1 | Mouse |
| Gene ID.1 | 243764 |
| Uniprot.1 | Q9ERZ4, |
| Species.2 | Rat |
| Gene ID.2 | 81645 |
| Uniprot.2 | P10980 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein . Phosphorylation in response to agonist binding promotes receptor internalization. . |
| Tissue Expression | Brain,Thalamus, |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Nervous system >> Cholinergic synapse;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>Calcium signaling pathway; / >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>PI3K-Akt signaling pathway; / >>Cholinergic synapse; / >>Regulation of actin cytoskeleton |
| Function | Disease:Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.,Function:The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.,polymorphism:Genetic variations in CHRM2 can influence susceptibility to alcoholism [MIM:103780].,similarity:Belongs to the G-protein coupled receptor 1 family., |
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