Price range: $268.00 through $328.00
Background：
MYL3 encodes myosin light chain 3 , an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq , Jul 2008] ,
| Size | 50μL/100μL |
| Target | MYL3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 22kD |
| MW(Observed) | 25kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:500; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1681R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MYL3 |
| Protein Name | Myosin light chain 3 |
| Other Name | MYL3; / Myosin light chain 3; / Cardiac myosin light chain 1; / CMLC1; / Myosin light chain 1; / slow-twitch muscle B/ventricular isoform; / MLC1SB; / Ventricular/slow twitch myosin alkali light chain |
| Species | Human |
| Gene ID | 4634 |
| Uniprot | P08590, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P09542 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | cytosol,muscle myosin complex,myosin complex,sarcomere,A band,I band, |
| Tissue Expression | Heart,Skeletal muscle, |
| Signaling_pathway | Organismal Systems >> Circulatory system >> Cardiac muscle contraction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Cardiac muscle contraction; / >>Adrenergic signaling in cardiomyocytes; / >>Apelin signaling pathway; / >>Hypertrophic cardiomyopathy; / >>Dilated cardiomyopathy |
| Function | Disease:Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.,Disease:Defects in MYL3 are the cause of cardiomyopathy hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.,Function:Regulatory light chain of myosin. Does not bind calcium.,PTM:The N-terminus is blocked.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains., |
