U-Blot® Pan-3-Nitrotyrosine Mouse mAb

$999.00

SKU: WT9176-300
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Product details

Background:
Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is very stable and so is suitable for analysis in biological sample such as plasma, lung aspirants-BALF (Broncho alveolar lining fluid) and urine. Increased nitrotyrosine levels have been associated with several physiological and pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Research shows that nitrotyrosine levels can be reduced by N-acetyl cysteine which is a precursor to glutathione, one of the body’s primary endogenous antioxidants.

Specifications

Target3-Nitrotyrosine
ReactivityHuman, Mouse, Rat
ApplicationWB、ICC/IF
MW(Calculated)Multiple
MW(Observed)Multiple
Host SpeciesMouse
IsotypeIgG
ConjugateUnconjugated
ModificationTyrosine nitration
Modified site
Recommended dilution ratioWB: 1:500–1:1000;ICC/IF: 1:100
CompositionPBS, Glycerol, BSA
Purification processProtein G-conjugated agarose followed by nitrotyrosine peptides agarose affinity chromatography
Purity
StorageStore at -20°C. Avoid repeated freeze-thaw cycles
StabilityStable for 12 months from date of receipt / reconstitution.
ClonalMonoclonal
Clone Number
Immunogenpolypeptide
Sequence
Specificity
Gene name
Protein Name
Alternative namenitro-Tyr
Organism-1
GeneID-1
SwissProt-1
Organism-2
GeneID-2
SwissProt-2
Organism-3
GeneID-3
SwissProt-3
Organism-4
GeneID-4
SwissProt-4
BackgroundNitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is very stable and so is suitable for analysis in biological sample such as plasma, lung aspirants-BALF (Broncho alveolar lining fluid) and urine. Increased nitrotyrosine levels have been associated with several physiological and pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Research shows that nitrotyrosine levels can be reduced by N-acetyl cysteine which is a precursor to glutathione, one of the body's primary endogenous antioxidants.
Cellular Localization
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