$999.00
Affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a methylated protein as the immunogen. The methyllysine specific antibodies are affinity purified with N-ε-mono-methyllysine and di-methyllysine on agarose as the affinity matrix. The purified antibody was conjugated to fluorescein. The conjugated antibody was tested to localize methylated proteins
| Target | Methyllysine |
| Reactivity | Species Independent |
| Application | IF |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | With fluorescein isothiocyanate (FITC) FITC/Ab molar ratio - 10:1 |
| Modification | Methylation |
| Modified site | |
| Recommended dilution ratio | IF 1:50 - 1:100 |
| Composition | 250 ug/mL in PBS, 50% glycerol |
| Purification process | N-e-mono-methyllysine and di-methyllysine on agarose |
| Purity | |
| Storage | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. |
| Stability | Expiration date is six months from date of shipping if properly stored. |
| Clonal | Polyclonal |
| Clone Number | |
| Immunogen | Methylated KLH conjugates |
| Sequence | |
| Specificity | |
| Gene name | |
| Protein Name | |
| Alternative name | |
| Organism-1 | |
| GeneID-1 | |
| SwissProt-1 | |
| Organism-2 | |
| GeneID-2 | |
| SwissProt-2 | |
| Organism-3 | |
| GeneID-3 | |
| SwissProt-3 | |
| Organism-4 | |
| GeneID-4 | |
| SwissProt-4 | |
| Background | Lysine methylation is a widespread and dynamically regulated post-translational modification. Methyl groups are covalently attached to the ε-amino group of lysine residues, forming mono-, di- and trimethylated lysine with distinct functional profiles. This modification does not alter protein charge, but changes steric hindrance and hydrophobicity, mediating interactions with downstream effector proteins. It is extensively involved in epigenetic regulation of chromatin structure and gene transcription, and also modulates protein stability, signal transduction and cell fate. Abnormal lysine methylation contributes to tumorigenesis, developmental defects and neurodegenerative diseases. |
| Cellular Localization |
