U-Blot® Pan-Ubiquitin Mouse mAb
Product details
Background:Ubiquitin conjugates to lysine (Lys, K) residues of a broad spectrum of cellular substrate proteins, including histones, cell cycle regulators, transcription factors, signaling molecules, apoptotic proteins, misfolded and damaged proteins. It also forms polyubiquitin chains via internal lysine sites (K6, K11, K27, K29, K33, K48, K63) to mediate protein degradation, DNA repair, gene transcription, cell cycle progression, apoptosis and immune signaling.
Specifications
| Target | Ubiquitin |
| Reactivity | Human, Mouse,Rat |
| Application | WB,ICC/IF |
| MW(Calculated) | Multiple |
| MW(Observed) | Multiple |
| Host Species | Mouse |
| Isotype | IgG2b/Kappa |
| Conjugate | Unconjugated |
| Modification | Ubiquitination |
| Modification Site | Lys(K) |
| Recommended dilution ratio | WB 1:500-1000;ICC/IF 1:100-200 |
| Composition | PBS, Glycerol, BSA |
| Purification process | Protein G |
| Storage | Store at -20°C. Avoid repeated freeze-thaw cycles |
| Stability | Stable for 12 months from date of receipt / reconstitution. |
| Concentration | |
| Clonal | Recombinant monoclonal |
| Clone Number | W-R10 |
| Immunogen | polypeptide |
| Gene Name | UBC, UBA52 |
| Protein Name | Ubiquitin |
| Alternative name | PUB C1, UBC4, UBC5B, UBCH4, UBCH5B |
| Organism-1 | Human |
| Gene ID | 7316 (UBC), 6232 (UBA52) |
| SwissProt-1 | P62979, P0CG47 |
| Cellular Localization | Cytosol, Extracellular region, Nucleus |
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