U-Blot® PCBP2 Rabbit mAb
Ucallm_WR6043-50
Price range: $268.00 through $328.00
Product details
Background：
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK , it is one of the major cellular poly (rC) -binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1 , this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES , promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA , human papillomavirus type 16 L2 mRNA , and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate
Specifications
| Size | 50μL/100μL |
| Target | PCBP2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 39kD |
| MW(Observed) | 40-45kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2035R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PCBP2 |
| Protein Name | Poly(rC)-binding protein 2 (Alpha-CP2) (Heterogeneous nuclear ribonucleoprotein E2) (hnRNP E2) |
| Other Name | |
| Species | Human |
| Gene ID | 5094 |
| Uniprot | Q15366, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q61990 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. |
| Tissue Expression | Detected in all tissues examined. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Ferroptosis |
| Research Areas | >>Ferroptosis |
| Function | Function:Major cellular poly(rC)-binding protein. Binds also poly(rU).,PTM:Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.,similarity:Contains 3 KH domains.,subcellular location:Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.,tissue specificity:Detected in all tissues examined., |
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