U-Blot® Phospho-HSP27 (Ser78) Rabbit mAb
Ucallm_WT9076-100
Price range: $288.00 through $368.00
Product details
Background:Hsp27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization
Specifications
| Target | Phospho-HSP27 (Ser78) |
| Reactivity | Human |
| Application | WB,IHC-P,ICC/IF,IP |
| MW(Calculated) | 23 kDa |
| MW(Observed) | 27 kDa |
| Host Species | Rabbit |
| Isotype | IgG/Kappa |
| Conjugate | Unconjugated |
| Modification | Phosphorylation |
| Modification Site | Ser78 |
| Recommended dilution ratio | WB 1:500-1:1000；IHC-P 1:100-1:500；ICC/IF 1:50；IP 1:50-1:100 |
| Composition | PBS, Glycerol, BSA |
| Purification process | Protein A affinity purification |
| Storage | Store at -20°C. Avoid repeated freeze-thaw cycles |
| Stability | Stable for 12 months from date of receipt / reconstitution. |
| Concentration | |
| Clonal | Recombinant monoclonal |
| Clone Number | W-R49 |
| Immunogen | polypeptide |
| Gene Name | HSPB1 |
| Protein Name | Heat shock protein 27 |
| Alternative name | Heat shock protein beta-1, HspB1, SRP27 |
| Organism-1 | Human |
| Gene ID | 3315 |
| SwissProt-1 | P04792 |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
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