U-Blot® Rec8 Rabbit mAb
Ucallm_WR6080-50
Price range: $268.00 through $328.00
Product details
Background：
This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse , the homologous protein is a key component of the meiotic cohesion complex , which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants , encoding the same protein , have been found for this gene. [provided by RefSeq , Jul 2008] ,
Specifications
| Size | 50μL/100μL |
| Target | Rec8 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 63kD |
| MW(Observed) | 83kD |
| Host Species | Rabbit |
| Isotype | IgG, Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1983R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | REC8 |
| Protein Name | Meiotic recombination protein REC8 homolog |
| Other Name | REC8; / REC8L1; / Meiotic recombination protein REC8 homolog; / Cohesin Rec8p |
| Species | Human |
| Gene ID | 9985 |
| Uniprot | O95072, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8C5S7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Chromosome . Chromosome , centromere . In meiotic chromosomes , localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages , diakinesis and metaphase I , localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. Localized to centromeres and spindle poles in endopolyploid tumor cells. . |
| Tissue Expression | Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level) . |
| Signaling_pathway | |
| Research Areas | >>Oocyte meiosis |
| Function | Function:Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. ,PTM:Phosphorylated. ,similarity:Belongs to the rad21 family. ,subcellular location:In meiotic chromosomes , localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages , diakinesis and metaphase I , localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. ,subunit:Interacts in both phosphorylated and unphosphorylated forms with SMC3 , SYCP3 and SMC1B but not with SMC1A. Also interacts with RAD51. ,tissue specificity:Expressed in testis and thymus. , |
Please login to post questions
Related products
