U-Blot® RNA pol II Monoclonal Antibody
UcallM
$999.00
Product details
Background:AbFlex ® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex ® RNA pol II CTD phospho Ser2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.
Specifications
| Target | -- |
| Reactivity | Budding Yeast, C. elegans, Human, Mouse, Rat |
| Applications | CUT&RUN; ChIP; ChIP-Seq; WB; IF; ICC |
| MW(Calculated) | 217 kDa |
| Host Species | Mouse |
| Isotype | IgG1 |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP: 10 µl per ChIP ChIP-Seq: 6-20 µl each WB: 1:2,000 - 1:5,000 dilution For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. |
| Formulation | PBS containing 0.2% gelatin, 0.01% thimerosal, 0.1% sodium azide, and 50% glycerol. Thimerosal and sodium azide are highly toxic. |
| Purification | Affinity Purified |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 4H8 |
| Immunogen | This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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